Anti-HSP47/SERPINH1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50454

Core Product Specifications and Parameters

Biological Background: HSP47/SERPINH1 Function and Localization

• SERPINH1 encodes serpin H1 (also known as HSP47, colligin, AsTP3, rheumatoid arthritis-related antigen RA-A47), a 46 kDa collagen-binding protein.
• Functions as a collagen-specific molecular chaperone, ensuring proper folding and assembly of procollagen in the endoplasmic reticulum.
• Plays a critical role in collagen biosynthesis, which is essential for the structural integrity of connective tissues, bone, and skin.
• Localized to the endoplasmic reticulum lumen, consistent with its role in post-translational processing of secretory proteins.
• Subject to multiple post-translational modifications including acetylation, phosphorylation, and N-glycosylation.
• Classified as a stress-response protein (heat shock protein), suggesting upregulation under cellular stress conditions.
• Mutations in SERPINH1 are associated with osteogenesis imperfecta, highlighting its importance in collagen-related pathologies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 319–418 of human HSP47; consider that epitope accessibility may vary across sample types.
• Western blotting (WB) on human or mouse lysates typically detects a band at ~46 kDa; use appropriate positive controls (e.g., fibroblast or osteoblast cell lines).
• For immunohistochemistry (IHC-P), antigen retrieval protocols may be required; validate in collagen-rich tissues such as dermis or bone.
• Immunofluorescence (IF/ICC) and ELISA applications have been reported; confirm assay conditions using recombinant protein or known positive samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supplying validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents for diagnostic development. For research on collagen biology or connective tissue disorders, we offer bulk procurement of this anti-HSP47 antibody along with rare-target raw material sourcing and technical support from concept to clinic.