Anti-HSP40 Rabbit Monoclonal Antibody for WB, FC, ELISA - P25685

Core Product Specifications and Parameters

Biological Background: HSP40/DNAJB1 Function and Localization

• Protein identity: DNAJB1 (also known as HSP40, Hdj1, HDJ-1) is a human molecular chaperone belonging to the DnaJ/HSP40 family, with two isoforms produced by alternative splicing.
• HSP70 co-chaperone activity: It binds HSP70 proteins and stimulates their ATPase activity, essential for protein folding and prevention of aggregation.
• Chaperone adapter function: Together with TTC1, it acts as a chaperone adapter that regulates HSP70-dependent folding by interacting with the HSP70 amino-terminal region (PubMed:14503850).
  Related references:
  PMID:14503850
• Heat shock response regulation: It negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase, modulating the cellular stress response (PubMed:9499401).
  Related references:
  PMID:9499401
• HSC70-HIP complex stabilization: DNAJB1 stimulates the association between the constitutive HSC70 chaperone and the co-chaperone HIP, enhancing protein quality control.
• ATP hydrolysis and folding catalysis: In vitro, it stimulates ATP hydrolysis and folding of unfolded proteins mediated by HSPA1A/B (HSP70-1), demonstrating direct chaperone activity (PubMed:24318877).
  Related references:
  PMID:24318877
• Subcellular localization: The protein is found in the cytoplasm, nucleus, and nucleolus, shuttling between these compartments under normal and stress conditions.
• Post-translational modifications: It is a phosphoprotein, and its activity may be regulated by phosphorylation; additional proteomics data confirm its identity.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a synthetic peptide within amino acids 241–340 of human DNAJB1, a region likely exposed in the native protein and conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• Western blotting (WB): For WB, use heat-shocked or stress-induced cell lysates as positive controls; expect bands near 27 kDa and 38 kDa (corresponding to different isoforms or modifications). Optimize loading and detection antibodies based on your sample.
• Flow cytometry (intracellular): For intracellular FC, permeabilization and fixation are required to detect cytoplasmic and nuclear HSP40; validate the staining with heat-shocked versus untreated cells.
• ELISA applications: This monoclonal antibody can be used as a capture or detection reagent in sandwich ELISA; appropriate pairing with a detection antibody (e.g., a polyclonal anti-HSP40) should be tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For heat shock protein and chaperone research, we supply validated monoclonal antibodies like this anti-HSP40 rabbit mAb, along with antibody pairs, ELISA kits, and bulk ancillary reagents. Our team also supports rare target sourcing, ensuring your HSP40/DNAJB1-related assays are fully equipped.