Anti-HSP27/HSPB1 Rabbit Monoclonal Antibody [KD Validated] for WB, IP, IF/ICC, IHC-P, ELISA - P04792

Core Product Specifications and Parameters

Biological Background: HSP27/HSPB1 Function and Localization

• HSPB1 (also known as HSP27, HSP28, SRP27) is a member of the small heat shock protein family, with a molecular weight of approximately 23 kDa.
• Functions as a molecular chaperone, maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Related references: PMID:10383393, PMID:20178975
• Plays a role in stress resistance and actin cytoskeleton organization, contributing to cellular protection under stress conditions (PubMed:19166925). Related references: PMID:19166925
• Through its chaperone activity, regulates biological processes including the phosphorylation and axonal transport of neurofilament proteins (PubMed:23728742). Related references: PMID:23728742
• Localizes to the cytoplasm, nucleus, and cytoskeleton (spindle) depending on cellular context and stress conditions.
• Highly expressed in all tissues, with highest levels in the heart and striated/smooth muscle tissues.
• Post-translational modifications include phosphorylation, acetylation, and methylation, which modulate its activity and function.
• Mutations in HSPB1 are associated with Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy type IIB (HMN2B).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-205 of human HSP27/HSPB1, corresponding to the full-length protein; this broad coverage may detect multiple post-translational modification states.
• For Western blot analysis, consider using a 10-12% SDS-PAGE gel to resolve the expected 23 kDa band, and validate detection using positive controls such as HeLa or MCF-7 cell lysates.
• The antibody is KD validated, making it a candidate for confirming siRNA or CRISPR-mediated HSPB1 knockdown experiments.
• In immunofluorescence applications, methanol or paraformaldehyde fixation may be explored to balance epitope preservation and cytoskeleton morphology.
• For immunohistochemistry, antigen retrieval conditions (e.g., citrate buffer pH 6.0) may need optimization based on tissue type and fixation method.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers researchers and diagnostic developers streamlined access to validated antibody raw materials, including this HSP27/HSPB1 Rabbit mAb, ideally suited for studying molecular chaperone mechanisms and stress-related pathways. Our one-stop platform supports your project from early-stage reagent procurement to clinical assay development, supplying validated antibody pairs, bulk monoclonal antibodies, and ancillary IVD raw materials. For specialized targets like HSPB1, we can also source rare research reagents to accelerate your diagnostic kit or biomarker discovery programs.