Anti-HSP27/HSPB1 Monoclonal Antibody for WB - P04792

Core Product Specifications and Parameters

Biological Background: HSP27/HSPB1 Function and Localization

• HSP27/HSPB1 is a small heat shock protein that functions as an ATP-independent molecular chaperone, binding to misfolded proteins and maintaining them in a folding-competent state. Related references: PMID:10383393 | PMID:20178975
• It plays a crucial role in stress resistance and actin cytoskeleton organization, contributing to cellular resilience under adverse conditions. Related references: PMID:19166925
• Through its chaperone activity, HSP27 may regulate the phosphorylation and axonal transport of neurofilament proteins, implicating it in neuronal function and maintenance. Related references: PMID:23728742
• The protein is also known as 28 kDa heat shock protein, estrogen-regulated 24 kDa protein, and stress-responsive protein 27 (SRP27).
• HSP27 is a member of the small heat shock protein family (HSPB) and is encoded by the HSPB1 gene (Gene ID: 3315) in humans.
• Subcellular localization includes the cytoplasm, nucleus, and cytoskeleton (spindle), reflecting its roles in protein homeostasis, signaling, and cell division.
• Post-translational modifications such as phosphorylation, acetylation, and methylation dynamically regulate its chaperone function and client interactions.
• Tissue expression is widespread, with highest levels in heart, skeletal muscle, and smooth muscle; it is also detectable in serum and cerebrospinal fluid, indicating both intracellular and extracellular roles.
• Clinically, mutations in HSPB1 are linked to Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy, underscoring its importance in neuronal health.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100–205 of human HSP27/HSPB1, a region that is highly conserved across human, mouse, and rat, which may contribute to the antibody's multi-species reactivity.
• For Western blot (WB) assays, the predicted molecular weight of HSP27 is ~23 kDa; however, post-translational modifications and sample conditions may cause band shifts; validating with appropriate controls is recommended.
• The antibody is suitable for immunofluorescence (IF/ICC and IF-P) applications, where HSP27 localization in the cytoplasm, nucleus, and cytoskeleton can be studied; fixation and permeabilization protocols should be optimized for each sample type.
• ELISA applications may be considered for quantifying HSP27 levels in various sample matrices; pairing with a secondary antibody and developing an appropriate standard curve would be necessary.
• Given the protein's involvement in stress responses and neurological disorders, researchers may consider inducing stress treatments (e.g., heat shock) or using disease-relevant cell lines and tissues for functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio—an integrated IVD raw-materials partner—supplies a comprehensive range of high-quality antibodies, antigen/antibody pairs, and ancillary reagents for research and diagnostic development. This HSP27/HSPB1 monoclonal antibody supports studies in stress biology, chaperone mechanisms, and neurodegenerative diseases such as CMT2F. Our one-stop sourcing bridge accelerates your transition from basic research to clinical assay development with tailored solutions for rare targets and bulk orders.