Anti-HSP20/HSPB6 Rabbit Polyclonal Antibody for WB, ELISA - O14558

Core Product Specifications and Parameters

Biological Background: HSP20/HSPB6 Function and Localization

• HSPB6, also known as heat shock protein beta-6 or Hsp20, is a small heat shock protein with a molecular weight of ~17 kDa, belonging to the HSPB family. It is encoded by the HSPB6 gene on chromosome 19 in humans.
• Functions as a molecular chaperone, likely maintaining denatured proteins in a folding-competent state, and is involved in the cellular stress response.
• Plays a critical role in regulating muscle function, including smooth muscle vasorelaxation and cardiac myocyte contractility.
• Overexpression of HSPB6 mediates cardioprotection and promotes angiogenesis after induced damage, possibly through regulation of the KDR (VEGFR2) pathway.
• Stabilizes monomeric YWHAZ (14-3-3 zeta), thereby supporting its chaperone-like activity and contributing to protein–protein interaction networks.
• Localizes to the cytoplasm and nucleus, and can also be secreted, as indicated by its presence in extracellular environments.
• HSPB6 is classified as a phosphoprotein, suggesting post-translational regulation via phosphorylation, and has been identified through direct protein sequencing and proteomics approaches.

Experimental Guidance and Technical Tips

• Consider using this antibody for Western blot detection of endogenous HSPB6 in human and mouse cell lysates; due to its polyclonal nature, it may recognize multiple epitopes across the 1–160 amino acid sequence.
• For ELISA applications, optimize coating and detection conditions; pairing with a matched capture antibody may enable sensitive sandwich assays.
• Validate the antibody in the relevant sample system and application, adjusting dilution factors and incubation times based on preliminary experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, offering this HSPB6 polyclonal antibody along with a comprehensive portfolio of antibodies, antigens, and ancillary reagents to support cardiovascular and stress-response research. We specialize in supplying validated antibody pairs, bulk monoclonal/polyclonal antibodies, and rare-target raw materials to accelerate your assay development and manufacturing. Partner with us for reliable, high-quality raw materials tailored to your diagnostic needs.