Anti-HSP20/HSPB6 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - O14558

Core Product Specifications and Parameters

Biological Background: HSP20/HSPB6 Function and Localization

• HSP20/HSPB6 (Heat shock protein beta-6) is a small heat shock protein that functions as a molecular chaperone, maintaining denatured proteins in a folding-competent state.
• It plays versatile roles in various biological processes, including regulating muscle function such as smooth muscle vasorelaxation and cardiac myocyte contractility.
• Overexpression of HSPB6 mediates cardioprotection and promotes angiogenesis after induced damage, potentially through regulation of KDR.
• HSPB6 stabilizes monomeric YWHAZ, thereby supporting YWHAZ chaperone-like activity.
• Subcellularly, it localizes to the cytoplasm and nucleus and is also secreted.
• The protein is classified under stress response and chaperone keywords and is a phosphoprotein regulated by post-translational modifications.
• It has a molecular weight of approximately 17 kDa (UniProt: 17136 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 70–150 of human HSP20/HSPB6 (O14558); thus, the antibody may recognize epitopes within this central region.
• For Western blot (WB), consider using reduced/denatured conditions and include appropriate positive controls (e.g., heart or muscle lysates) where HSPB6 is expressed.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), verify permeabilization and fixation conditions compatible with epitope accessibility.
• For ELISA, validate the antibody with recombinant or native HSPB6 protein in a standard direct or sandwich assay format.
• Since cross-reactivity is reported for human, mouse, and rat, the antibody may be suitable for experiments in these species; however, always confirm specificity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing validated raw materials, technical services, and consulting. For HSP20/HSPB6, we offer monoclonal antibodies and can assist with sourcing rare-target reagents, ancillary reagents, and custom IVD development needs. From concept to clinic, CamelBio is your trusted partner for stress response and cardioprotection research tools.