Anti-HSP105/HSPH1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q92598

Core Product Specifications and Parameters

Biological Background: HSP105/HSPH1 Function and Localization

• Also known as heat shock 110 kDa protein, antigen NY-CO-25, and heat shock protein family H member 1.
• Functions as a nucleotide-exchange factor (NEF) for HSPA1A and HSPA1B, promoting ADP release and client/substrate protein release. Related references: PMID:24318877
• Prevents aggregation of denatured proteins under severe stress with low ATP levels (By similarity).
• Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity).
• Located in the cytoplasm.
• Highly expressed in testis; lower levels in most brain regions except cerebellum; overexpressed in cancer cells.
• Subject to acetylation and phosphorylation; alternative splicing generates isoforms.
• Classified as an ATP-binding, nucleotide-binding phosphoprotein involved in stress response; has a characterized 3D structure and is identified in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is ~97 kDa; validate with a positive control (e.g., heat-shocked HeLa or cancer cell lines).
• In immunohistochemistry (paraffin), perform antigen retrieval and confirm cytoplasmic staining pattern.
• For ELISA, the immunogen covers the C-terminal region (aa 659-858); this region may be suitable for capture/detection sandwich design.
• As this is a polyclonal antibody, lot-to-lot variability should be assessed and optimal dilutions determined experimentally.
• To enhance detection of HSP105, consider inducing cellular stress (e.g., heat shock) in sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive range of IVD raw materials, including validated antibodies like this anti-HSP105/HSPH1 polyclonal antibody for stress response and chaperone studies. Beyond individual reagents, we offer antibody pair development, bulk ancillary reagents, and sourcing for rare targets, streamlining your path from concept to clinical application.