Anti-HSP105/HSPH1 Rabbit Monoclonal Antibody for WB and ELISA - Q92598

Core Product Specifications and Parameters

Biological Background: HSP105/HSPH1 Function and Localization

• Heat shock protein 105 kDa (HSP105), also known as HSP110 or antigen NY-CO-25, is encoded by the HSPH1 gene and belongs to the HSP110 family. The protein exists in multiple isoforms due to alternative splicing.
• HSP105 functions as a nucleotide-exchange factor (NEF) for the chaperones HSPA1A and HSPA1B, promoting ADP release and facilitating client protein release (PubMed:24318877). Related references: PMID:24318877
• Under severe cellular stress with depleted ATP, HSP105 prevents the aggregation of denatured proteins, thereby maintaining protein homeostasis.
• Additionally, HSP105 inhibits the ATPase and chaperone activities of HSC70 (HSPA8), modulating the chaperone network.
• Subcellularly, HSP105 localizes predominantly to the cytoplasm, consistent with its role in stress response.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and interactions.
• HSP105 is highly expressed in the testis and at lower levels in most brain regions except the cerebellum; it is frequently overexpressed in various cancer cells, linking it to oncogenic processes.

Experimental Guidance and Technical Tips

• The antibody was generated using a synthetic peptide corresponding to the N-terminal region (aa 1-100). It is predicted to detect full-length HSP105 and potentially N-terminal isoforms.
• For Western Blot, consider optimizing the primary antibody dilution (e.g., 1:500–1:2000) based on your sample type and detection system. Include appropriate positive and negative controls.
• Due to cross-reactivity with mouse and rat, this antibody can be used for comparative studies across these species; however, validation in the specific tissue or cell line of interest is recommended.
• ELISA applications may benefit from standard checkerboard titrations to determine the optimal coating and antibody concentrations.
• As a monoclonal antibody, it offers consistent lot-to-lot performance, making it suitable for reproducible assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories a streamlined sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-HSP105/HSPH1 antibody aligns with research on heat shock proteins, cellular stress responses, and cancer biology. Whether you need ready-to-use immunoreagents or support in rare target raw-material procurement, CamelBio serves as your reliable partner from concept to clinic.