Anti-HSL Rabbit Polyclonal Antibody for WB, ELISA - Q05469

Core Product Specifications and Parameters

Biological Background: Hormone-sensitive lipase Function and Localization

• Hormone-sensitive lipase (HSL), encoded by the LIPE gene, is a multifunctional enzyme with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters, and retinyl esters. It shows preferential hydrolysis of DAGs over TAGs and MAGs, and preferentially hydrolyzes the sn-3 position in DAGs and sn-1/sn-2 positions in TAGs.

Related references: PMID:15716583, PMID:15955102, PMID:19800417

• HSL hydrolyzes 2-arachidonoylglycerol, an endocannabinoid, and 2-acetyl monoalkylglycerol ether, the penultimate precursor of platelet-activating factor, linking HSL to endocannabinoid signaling and inflammatory mediator synthesis.

• In adipose tissue and heart, HSL primarily releases free fatty acids from stored triglycerides for energy; in steroidogenic tissues, it converts cholesteryl esters to free cholesterol for steroid hormone production.

• Subcellularly, HSL localizes to the cell membrane, caveolae, cytoplasm, cytosol, and the surface of lipid droplets, reflecting its dynamic role in lipid mobilization.

• Tissue expression is notably high in testis, but HSL is functionally important in multiple metabolic tissues.

• As a key lipid hydrolase, HSL is implicated in obesity, diabetes mellitus, and cholesterol/steroid metabolism disorders.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 900-1000), ensuring specific recognition of HSL. For Western blot, expect a band at approximately 117 kDa; validate using adipose tissue or other HSL-expressing cell lysates.

• For ELISA, consider using the immunizing peptide or full-length recombinant HSL protein to optimize assay conditions and antibody titration.

• As a rabbit polyclonal antibody, lot-to-lot variability may occur; each new lot should be validated in your specific sample matrix and application.

• Cross-reactivity with mouse and rat indicates suitability for rodent models; include appropriate species-matched controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, supporting diagnostic developers and research labs across the entire product lifecycle. Our HSL/LIPE antibody portfolio enables reliable detection in metabolic disease studies, and we offer an extensive range of validated antibody pairs, optimized polyclonal and monoclonal antibodies, recombinant antigens, and bulk ancillary reagents for lipid metabolism research. Contact us to streamline your sourcing of rare-target materials and accelerate your assay development.