Anti-HSF2 Rabbit pAb for WB, ELISA - Q03933

Core Product Specifications and Parameters

Biological Background: HSF2 Function and Localization

• HSF2 is a heat shock transcription factor that specifically binds to heat shock promoter elements (HSE) to activate transcription.
• In higher eukaryotes, HSF2 binding to HSE requires cellular stress, such as heat shock, for activation.
• The protein localizes to both the cytoplasm and nucleus, shuttling between compartments in response to stress signals.
• Alternative splicing of the HSF2 gene generates isoforms that may have distinct regulatory roles.
• HSF2 is involved in the cellular stress response, regulating the expression of heat shock proteins and other protective genes.
• Post-translational modifications, including ubiquitination, modulate HSF2 activity and stability.
• Experimental evidence from direct protein sequencing and proteomics supports the existence and expression of HSF2.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant fusion protein covering the N-terminal DNA-binding region (aa 39-215). It may recognize multiple HSF2 isoforms.
• For Western blot applications, expect a band at approximately 60 kDa in lysates from human, mouse, and rat cells. Validate and optimize dilution in your sample system.
• For ELISA, the antibody can be used for detection or capture; appropriate pairing and titration are recommended.
• Cross-reactivity with human, mouse, and rat is confirmed; additional species should be tested if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibodies, antigens, and ancillary reagents for diagnostic development. The HSF2 Rabbit pAb is suitable for research into cellular stress, cancer biology, and transcriptional regulation, supporting your assay development from concept to clinic. For bulk orders, customized antibody pairs, or rare target sourcing, contact CamelBio as your trusted supply partner.