Anti-HSF1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q00613

Core Product Specifications and Parameters

Biological Background: HSF1 Function and Localization

• HSF1 (heat shock factor protein 1), also known as HSTF1, is a stress-inducible transcription factor pivotal in the heat shock response, promoting expression of heat shock proteins (HSPs) to safeguard cells from proteotoxic stress. Related references: PMID:11447121 PMID:12659875 PMID:12917326
• In the absence of stress, HSF1 is held in an inactive monomeric state through interaction with an HSP90-containing multichaperone complex. Upon heat shock, it undergoes homotrimerization, binds to heat shock elements (HSEs) on DNA, and activates HSP gene transcription. Related references: PMID:9727490 PMID:11583998 PMID:16278218 PMID:10359787
• HSF1 recognizes inverted 5'-NGAAN-3' pentamer sequences and localizes to chromatin at heat shock gene promoters, forming a complex with TTC5 and p300 to stimulate transcription. Related references: PMID:1986252 PMID:26727489 PMID:25963659 PMID:18451878
• Besides transcriptional activation, HSF1 modulates post-transcriptional processes: it positively regulates pre-mRNA 3'-end processing/polyadenylation and nuclear export of HSP70 mRNA, in part via symplekin (SYMPK). Related references: PMID:14707147 PMID:17897941
• HSF1 exhibits transcription-independent functions including repression of Ras‑induced c‑fos expression, regulation of mitotic progression, and negative control of non‑homologous end joining (NHEJ) DNA repair. Related references: PMID:9341107 PMID:18794143 PMID:26359349
• Subcellularly, HSF1 is detected in the nucleus, nucleoplasm, cytoplasm, perinuclear region, spindle pole, centrosome, and kinetochore, reflecting its multifaceted roles.
• Post‑translational modifications such as phosphorylation and acetylation fine‑tune HSF1 activity, while its interaction with viral elements, like the HIV‑1 LTR, underscores its role in host‑pathogen interactions.

Experimental Guidance and Technical Tips

• The immunogen (aa 350‑529) targets the C‑terminal portion of HSF1, which is less conserved than the DNA‑binding domain; confirm specificity using lysates from heat‑shocked versus untreated cells.
• For Western blotting, a band around 57 kDa is expected; include a heat‑shocked positive control (e.g., HeLa cells treated at 42°C for 1 h) and a loading control.
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), optimize antigen retrieval and fixation conditions; HSF1 nuclear localization increases upon stress, so consider comparing stressed and unstained tissue sections.
• As a polyclonal antibody, lot‑to‑lot consistency may vary; titrate for each application and run appropriate controls, especially when switching to a new lot.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering reliable access to validated antibodies like this anti‑HSF1 polyclonal antibody for heat shock and stress research. Beyond single reagents, CamelBio supports bulk procurement of antibody pairs, ancillary reagents, and can assist in sourcing rare‑target materials to streamline your development pipeline.