Anti-HSF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q00613

Core Product Specifications and Parameters

Biological Background: HSF1 Function and Localization

• HSF1 is a stress-inducible transcription factor that master-regulates the heat shock response (HSR) by driving expression of molecular chaperones and heat shock proteins (HSPs) (PubMed:11447121, PubMed:12659875, PubMed:12917326).
  Related references: PMID:11447121, PMID:12659875, PMID:12917326
• In unstressed cells, HSF1 is maintained as an inactive monomer in a complex with HSP90; upon stress, it homotrimerizes, translocates to the nucleus, and activates transcription (PubMed:11583998, PubMed:16278218, PubMed:9727490).
  Related references: PMID:11583998, PMID:16278218, PMID:9727490
• HSF1 binds to inverted 5'-NGAAN-3' pentamer DNA sequences and associates with chromatin at heat shock gene promoters to drive gene expression (PubMed:1986252, PubMed:26727489, PubMed:25963659).
  Related references: PMID:1986252, PMID:26727489, PMID:25963659
• Beyond transcription, HSF1 participates in mRNA processing and nuclear export of HSP70 transcripts, and regulates mitotic progression and DNA damage repair via non-homologous end joining (NHEJ) repression (PubMed:14707147, PubMed:17897941, PubMed:18794143, PubMed:26359349).
  Related references: PMID:14707147, PMID:17897941, PMID:18794143, PMID:26359349
• HSF1 is subject to extensive post-translational modifications including acetylation, phosphorylation, and ubiquitination, which modulate its activity and stability.
• In HIV-1 infection, HSF1 can reactivate latent viral transcription by binding the LTR and recruiting elongation factors CDK9 and CCNT1.
• HSF1 localizes dynamically to the nucleus, cytoplasm, spindle pole, centrosome, and kinetochore, reflecting its diverse cellular functions.
• The human HSF1 gene (GeneID: 3297) produces multiple isoforms via alternative splicing; the predominant form migrates at ~57 kDa, and an alternative isoform is observed at ~52 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 367-529) targets the C-terminal region of HSF1, which includes transactivation and regulatory domains, ensuring recognition of full-length and potential isoforms.
• For WB, expect bands near 52–57 kDa; consider using lysates from heat-shocked HeLa or Jurkat cells as positive controls, and validate with knockdown or negative cell lines.
• In IHC-P experiments, optimize antigen retrieval (e.g., citrate buffer pH 6.0) and validate staining patterns in known HSF1-expressing tissues (e.g., testis) or heat-stressed samples.
• For ELISA, use recombinant HSF1 protein to confirm antibody binding and determine the optimal working concentration through checkerboard titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with premium IVD raw materials, offering this anti-HSF1 polyclonal antibody for heat shock and oncology applications. Our portfolio includes validated antibody pairs, bulk custom antibody production, and rare-target sourcing to streamline your assay development. Partner with CamelBio for reliable, end-to-end support from concept to clinic.