Anti-HSF1 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q00613

Core Product Specifications and Parameters

Biological Background: HSF1 Function and Localization

• HSF1 is the heat shock factor protein 1 (HSTF1), a stress-inducible transcription factor that activates heat shock protein (HSP) gene expression to protect cells from stress-induced damage.

Related references: PMID:11447121, PMID:12659875, PMID:12917326

• In unstressed cells, HSF1 is maintained in a monomeric, DNA-binding-inactive state through association with an HSP90-containing multichaperone complex.

Related references: PMID:11583998, PMID:16278218, PMID:9727490

• Upon stress, HSF1 undergoes homotrimerization and binds to heat shock elements (HSEs) in HSP gene promoters. It can also form a co-activator complex with TTC5/STRAP and p300 to enhance transcription.

Related references: PMID:10359787, PMID:11583998, PMID:12659875, PMID:18451878

• HSF1 specifically recognizes inverted 5'-NGAAN-3' pentamer sequences and localizes to chromatin at HSP gene promoters.

Related references: PMID:1986252, PMID:26727489, PMID:25963659

• Beyond transcriptional regulation, HSF1 participates in mRNA 3'-end processing and nuclear export of HSP70 mRNA, and contributes to mitotic progression control.

Related references: PMID:14707147, PMID:17897941, PMID:18794143

• HSF1 negatively regulates non-homologous end joining (NHEJ) DNA repair and plays a role in HIV-1 latency by reactivating viral transcription from the LTR promoter.

Related references: PMID:26359349

• Subcellularly, HSF1 localizes to the nucleus, cytoplasm, perinuclear region, centrosome, spindle pole, centromere, and kinetochore, reflecting its diverse functions.

• Post-translational modifications including acetylation, phosphorylation, and ubiquitination regulate HSF1 activity, stability, and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 429–530) of human HSF1. Consider this region’s conservation and potential isoform expression when designing your experiments.
• Validated applications: WB, IHC-P, IP, ELISA. Optimize antibody dilution, incubation times, and detection conditions for your specific sample type and assay format.
• This antibody cross-reacts with human, mouse, and rat HSF1. For other species, perform a validation experiment to confirm reactivity.
• The predicted molecular weight is approximately 57 kDa. Post-translational modifications may alter migration; include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-HSF1 rabbit monoclonal antibody as part of a comprehensive portfolio of IVD raw materials for heat shock response, stress biology, and transcription research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets to support your assay development from early concept to clinical application.