Anti-HSD3B2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P26439

Core Product Specifications and Parameters

Biological Background: HSD3B2 Function and Localization

• HSD3B2 (3-beta-HSD II) is encoded by the HSD3B2 gene and belongs to the 3-beta-hydroxysteroid dehydrogenase/isomerase family. It is predominantly expressed in the adrenal gland, testis, and ovary, where it catalyzes a key step in steroid hormone biosynthesis.
• The enzyme functions as a bifunctional catalyst: it oxidizes Delta(5)-ene-3-beta-hydroxy steroids to Delta(4)-ene-3-keto steroids and then isomerizes them to active hormonal steroids. This activity is essential for the production of progesterone, glucocorticoids, mineralocorticoids, and sex steroids.
• HSD3B2 is localized to both the endoplasmic reticulum membrane and the mitochondrial membrane, with transmembrane domains anchoring it to these organelles. This dual localization supports its role in multiple steps of steroidogenesis.
• Clinical significance: Mutations in HSD3B2 are associated with congenital adrenal hyperplasia (CAH), a disorder characterized by impaired cortisol and aldosterone synthesis, leading to adrenal insufficiency and variable degrees of sexual ambiguity.
• The antibody's immunogen covers amino acids 1-287 of the full-length protein (reference sequence NP_000189.1), which encompasses the catalytic domain, ensuring recognition of the native enzyme in human and mouse samples.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 42 kDa is expected in lysates from human adrenal, testis, or ovary cell lines or tissues. Mouse samples with confirmed expression can also be used, but the antibody's reactivity in mouse should be validated in your specific system.
• In immunofluorescence/ICC, anticipate staining patterns consistent with endoplasmic reticulum and mitochondrial localization. Use organelle-specific markers and appropriate permeabilization methods to help confirm subcellular distribution.
• As a polyclonal antibody, it may provide stronger signal due to multiple epitope recognition, but lot-to-lot variability can occur. Consider including a known positive control (e.g., recombinant HSD3B2 protein) in each experiment.
• For ELISA, the antibody can be used as a capture or detection reagent, but optimal dilutions and pairing conditions must be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, providing validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw materials. For research on steroidogenesis, endocrine disorders, and hormone biosynthesis, we supply optimized antibody pairs and reliable reagents to support diagnostic development from concept to clinic. Contact us for tailored sourcing solutions that accelerate your assay development.