Anti-HSD3B1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P14060

Core Product Specifications and Parameters

Biological Background: HSD3B1 Function and Localization

• HSD3B1, also known as 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (3β-HSD I), is encoded by the HSD3B1 gene (aliases: HSDB3A, 3BH, SDR11E1).
• It is a bifunctional enzyme essential for steroid hormone biosynthesis, catalyzing the oxidation and isomerization of 3β-hydroxy-Δ5-steroid precursors to 3-oxo-Δ4-steroids. Related references: PMID:1401999 PMID:2139411
• Specifically converts pregnenolone to progesterone, 17α-hydroxypregnenolone to 17α-hydroxyprogesterone, DHEA to androstenedione, and androstenediol to testosterone.
• Also interconverts 3β-hydroxy and 3-oxo-5α-androstane steroids, controlling active androgen bioavailability; for example, it converts dihydrotestosterone (DHT) to inactive 5α-androstanediol.
• Subcellular localization includes the endoplasmic reticulum membrane and mitochondrial membrane.
• Highly expressed in placenta, skin, and mammary gland tissue.
• Classified under keywords: multifunctional enzyme, steroidogenesis, lipid metabolism, NAD/NADP, and transmembrane.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250–350 of human HSD3B1; therefore, the antibody likely recognizes an internal epitope and may be sensitive to conformational changes.
• For Western blotting, a band near 42 kDa is expected. Validate detection in the relevant sample system and consider using appropriate positive controls (e.g., placental or mammary tissue lysates).
• In IHC-P and ELISA, antigen retrieval and blocking conditions may need optimization. The antibody cross-reacts with mouse and rat, enabling multi-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This HSD3B1 monoclonal antibody supports research into steroidogenesis and hormone-related disorders. For rare target raw-material sourcing or custom development, CamelBio’s comprehensive supply chain and technical support accelerate your journey from concept to clinic.