Anti-HSD3B1 Polyclonal Antibody for WB, IF-P, ELISA - P14060

Core Product Specifications and Parameters

Biological Background: HSD3B1 Function and Localization

• HSD3B1 is a bifunctional enzyme belonging to the 3-beta hydroxysteroid dehydrogenase/isomerase family. It catalyzes the oxidation and isomerization of 3β-hydroxy-Δ5-steroid precursors to 3-oxo-Δ4-steroids, an essential step in the biosynthesis of all major steroid hormones. This includes the conversion of pregnenolone to progesterone, 17α-hydroxypregnenolone to 17α-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Related references: PMID:1401999 PMID:2139411
• Beyond de novo steroidogenesis, HSD3B1 regulates local androgen bioavailability by interconverting active and inactive 5α-reduced androgens. It efficiently inactivates dihydrotestosterone (DHT) by converting it to 5α-androstanediol, a metabolite that does not bind the androgen receptor. Related references: PMID:1401999 PMID:2139411
• HSD3B1 is anchored to intracellular membranes via a single-pass transmembrane helix and localizes to both the endoplasmic reticulum membrane and the mitochondrial membrane.
• The enzyme is predominantly expressed in placenta and skin, and is also found at significant levels in mammary gland tissue.
• It is classified under multiple functional keywords, including multifunctional enzyme, NAD/NADP-dependent oxidoreductase, isomerase, steroid metabolism, and lipid metabolism, highlighting its central role in steroidogenic and metabolic pathways.
• According to UniProt, HSD3B1 is expected to use NAD+ as the preferred electron donor for its dehydrogenase activity and NADPH for the reductase activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human HSD3B1 protein (amino acids 1-290), which may generate a polyclonal response targeting multiple epitopes. For Western blot, consider using positive control lysates from placental or mammary tissue, or cell lines with high endogenous HSD3B1 expression (e.g., breast or prostate cancer cells).
• The predicted molecular weight is approximately 42 kDa. Due to its membrane localization, ensure sufficient solubilization by using appropriate lysis buffers containing detergents (e.g., RIPA buffer) and avoid boiling samples at excessively high temperatures to prevent aggregation.
• For immunofluorescence (IF-P), include a permeabilization step (e.g., 0.1% Triton X-100) to allow antibody access to intracellular membrane compartments. Co-staining with ER or mitochondrial markers can validate the expected subcellular localization.
• The antibody has confirmed cross-reactivity with human, mouse, and rat samples. Verify suitability for other species by checking sequence homology and performing a pilot experiment.
• As a polyclonal antibody, lot-to-lot consistency may vary. Consider aliquot storage and gradient testing when optimizing for new assays, and validate performance in your own sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to empowering diagnostic manufacturers and research teams with comprehensive access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This HSD3B1 polyclonal antibody serves as a reliable tool for studies in endocrinology, reproductive biology, and cancer metabolism. Through our integrated supply network, we help you source rare-target antibodies, optimized monoclonals, and customized production support—bridging the gap from concept to clinic.