Anti-HSD17B8 Polyclonal Antibody for WB, IF/ICC, ELISA - Q92506

Core Product Specifications and Parameters

Biological Background: HSD17B8 Function and Localization

• HSD17B8 (17-beta-hydroxysteroid dehydrogenase 8), also known as KE6, FABG, and SDR30C1, is a 27 kDa member of the short-chain dehydrogenase/reductase (SDR) family.
• Acts as the alpha subunit of the mitochondrial 3-ketoacyl-ACP reductase (KAR) complex, serving as a scaffold protein essential for the solubility and stability of the beta subunit CBR4, thereby enabling mitochondrial fatty acid biosynthesis. Related references: PMID:19571038, PMID:25203508, PMID:30508570
• Catalyzes the NAD-dependent conversion of (3R)-3-hydroxyacyl-CoA to 3-ketoacyl-CoA with no chain length preference; cooperates with enoyl-CoA hydratase 1 as an alternative route for cis-polyunsaturated fatty acid breakdown. Related references: PMID:19571038, PMID:30508570
• Functions as a 17-beta-hydroxysteroid dehydrogenase with highest activity toward estradiol, exhibiting primarily oxidative but conditionally reductive activity to convert estrone to estradiol. Related references: PMID:17978863
• Localized to the mitochondrial matrix.
• Widely expressed, with high abundance in prostate, placenta, and kidney; detected at protein level in brain, cerebellum, heart, lung, ovary, testis, and adrenals. Related references: PMID:17978863, PMID:30508570
• Identified as a phosphoprotein with a known 3D structure; participates in fatty acid and steroid biosynthesis.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering full-length human HSD17B8 (aa 1–261); this design may detect all described isoforms.
• For Western blot, a band at ~27 kDa is expected; verify specificity using mitochondrial lysates from human, mouse, or rat tissues.
• In immunofluorescence/ICC, expect a mitochondrial staining pattern; include appropriate controls and validate in relevant cell systems.
• For ELISA applications, optimize antigen coating and antibody dilution empirically; cross-reactivity with mouse and rat homologs can aid multi-species assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with reliable IVD raw materials, including validated antibody pairs, tailored monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects targeting mitochondrial fatty acid metabolism or steroid hormone pathways, we streamline procurement of anti-HSD17B8 antibodies and complementary tools—supporting your workflow from early research to clinical application.