Anti-HSD17B7 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P56937

Core Product Specifications and Parameters

Biological Background: HSD17B7 Function and Localization

• HSD17B7 (also known as PRAP, SDR37C1) is a bifunctional enzyme catalyzing 3‑ketosteroid reduction and 17β‑hydroxysteroid dehydrogenase activity, linking steroid hormone metabolism and cholesterol biosynthesis.
  Related references: PMID:11165030, PMID:12574203, PMID:12732193
• It reduces estrone (E1) to the more potent 17β‑estradiol (E2) and inactivates dihydrotestosterone (DHT) to 5α‑androstane‑3β,17β‑diol, modulating estrogen and androgen signaling.
  Related references: PMID:12574203, PMID:12732193, PMID:19772289
• The enzyme participates in the post‑squalene cholesterol biosynthesis pathway by reducing zymosterone and other intermediates.
  Related references: PMID:11165030, PMID:12829805, PMID:20659585
• HSD17B7 is an integral membrane protein of the endoplasmic reticulum, with a single transmembrane domain.
• It is widely expressed, with highest levels in adrenal gland, liver, lung, and thymus; also found in reproductive tissues, kidney, and the eye ciliary epithelium.
• The protein is subject to alternative splicing and N‑glycosylation, which may regulate its activity or localization.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 110–200) lies within the catalytic domain; as a polyclonal antibody, it may recognize multiple epitopes, including isoforms.
• For Western blot, the predicted molecular weight is 38 kDa; consider that post‑translational modifications (glycosylation) or alternative splicing may shift the observed band.
• In immunohistochemistry (IHC‑P), validate with known positive tissues (e.g., adrenal gland, liver) and include appropriate controls due to potential cross‑reactivity with other short‑chain dehydrogenase/reductase family members.
• For ELISA, the antibody can be used as capture or detection reagent; performance should be titrated in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable one-stop channel for IVD raw materials. As a supplier of this anti‑HSD17B7 polyclonal antibody, we also offer validated antibody pairs, optimized recombinant proteins, and ancillary reagents to accelerate your steroid metabolism or cholesterol biosynthesis assay development. Contact us for bulk orders, custom conjugation, and assistance with sourcing rare targets.