Anti-HSD17B3 Rabbit Polyclonal Antibody for WB and ELISA - P37058

Core Product Specifications and Parameters

Biological Background: HSD17B3 Function and Localization

• HSD17B3 (17-beta-hydroxysteroid dehydrogenase type 3) catalyzes the conversion of 17-oxosteroids to 17beta-hydroxysteroids, favoring the reduction of androstenedione to testosterone. Related references: PMID:16216911, PMID:23856005, PMID:26545797
• Testosterone produced by HSD17B3 is the key androgen driving male development and function. Related references: PMID:8075637
• The enzyme uses NADPH as a cofactor, distinguishing it from other EDH17B enzymes that use NADH. Related references: PMID:16216911, PMID:26545797, PMID:8075637
• It accepts various androgen substrates such as epiandrosterone, dehydroepiandrosterone, androsterone, and androstanedione, and can reduce 11-ketoandrostenedione and 11beta-hydroxyandrostenedione to testosterone forms. Related references: PMID:16216911, PMID:27927697
• HSD17B3 is localized to the endoplasmic reticulum and is predominantly expressed in the testis.
• Mutations in HSD17B3 are associated with pseudohermaphroditism, highlighting its critical role in male sexual development.
• Alternative splicing of the gene generates multiple transcript variants, and the protein is identified in proteomics studies.
• As an oxidoreductase involved in steroid biosynthesis, it plays a key role in lipid metabolism.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell lysates from testis or cells expressing HSD17B3, as the protein is testis-specific.
• The antibody recognizes amino acids 1-310, so it may detect both full-length and truncated isoforms; validate in the relevant sample system.
• Cross-reactivity with mouse and rat suggests conserved epitopes; suitable for comparative studies.
• For ELISA, optimize coating antigen and detection conditions with the recombinant protein or peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials like validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our global sourcing capabilities cover rare and specialized targets such as HSD17B3 for steroidogenesis and androgen signaling research, ensuring you find the right tools for your assay development. Trust CamelBio to bridge supply gaps from concept to clinic with reliable, research-use products and expert procurement support.