Anti-HSD17B2 Monoclonal Antibody for WB, IHC-P, ELISA - P37059

Core Product Specifications and Parameters

Biological Background: HSD17B2 Function and Localization

• HSD17B2 (also known as EDH17B2, SDR9C2, 20-alpha-HSD) is a member of the short-chain dehydrogenase/reductase superfamily.
• It functions as a 17-beta-hydroxysteroid dehydrogenase type 2, oxidizing highly active 17beta-hydroxysteroids (estradiol, testosterone, DHT) to their less active forms, thereby regulating steroid hormone potency.
• Also exhibits 20-alpha-hydroxysteroid dehydrogenase activity.
• Localizes to the endoplasmic reticulum membrane via a single transmembrane helix (signal-anchor).
• Participates in NAD-dependent oxidoreductase activity, essential for steroid biosynthesis and lipid metabolism.
• Expressed in placenta, suggesting a role in modulating active estrogen and androgen levels during pregnancy.
• Acts as a key enzyme in intracellular steroid hormone inactivation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic domain of HSD17B2, supporting recognition of the native protein in denaturing conditions (WB) and fixed tissue (IHC-P).
• For WB, expect a single band around 43 kDa; validate size with appropriate molecular weight markers.
• For IHC-P, consider standard antigen retrieval protocols and validate staining specificity in relevant tissues (e.g., placenta).
• ELISA performance should be verified using recombinant HSD17B2 protein as the coating antigen.
• Cross-reactivity with mouse and rat is predicted due to immunogen sequence conservation; confirm in your assay system.

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