Anti-HSD17B13 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q7Z5P4

Core Product Specifications and Parameters

Biological Background: HSD17B13 Function and Localization

• 17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13), also known as hepatic retinol/retinal dehydrogenase, SDR16C3, and SCDR9, belongs to the short-chain dehydrogenase/reductase family.
• Plays a pivotal role in hepatic lipid metabolism (PubMed:29562163). Related references: PMID:29562163
• In vitro, catalyzes the oxidation of various lipid substrates, including 17beta-estradiol, retinol, retinal, and leukotriene B4 (PubMed:29562163, PubMed:30415504, PubMed:32973038). Related references: PMID:29562163, PMID:30415504, PMID:32973038
• Conflicting reports exist regarding its retinol/retinal dehydrogenase activity; some studies indicate activity, while others do not.
• Localizes to lipid droplets, the endoplasmic reticulum, and the cytoplasm.
• Highly expressed in the liver, with additional expression in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder, and testis.
• Classified as a phosphoprotein and undergoes alternative splicing; a 3D structure has been determined.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 34-300 of human HSD17B13, a region containing the catalytic domain; this may provide broad reactivity across isoforms.
• For Western blotting, consider using a 1:500-1:2000 dilution range and validate with positive control lysates (e.g., HepG2 or liver tissue).
• In immunofluorescence/ICC, check for lipid droplet and ER co-localization; include detergent permeabilization to detect cytoplasmic pools.
• For ELISA, pairing with a validated capture antibody may improve sensitivity; optimize the antigen coating concentration and detection antibody dilution.
• Cross-reactivity with rat HSD17B13 is indicated; verify specificity in your experimental system using species-matched controls.
• Since commercial antibodies may show batch variability, validate lot-to-lot consistency for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material supplier, CamelBio connects you with high-quality reagents for every stage of diagnostic development. This anti-HSD17B13 antibody supports liver metabolism research, and we can further source optimized monoclonal/polyclonal antibodies, validated antibody pairs, and ancillary reagents in bulk. Leverage our global network to streamline your procurement of rare-target or custom-made raw materials.