Anti-HSD17B13 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q7Z5P4

Core Product Specifications and Parameters

Biological Background: HSD17B13 Function and Localization

• HSD17B13 is a 34 kDa protein belonging to the short-chain dehydrogenase/reductase (SDR) family, also designated as SDR16C3 or hepatic retinol/retinal dehydrogenase.
• It is highly expressed in the liver and also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder, and testis. Related references: PMID:29562163
• HSD17B13 localizes primarily to lipid droplets and the endoplasmic reticulum, with some cytoplasmic presence.
• It plays a critical role in hepatic lipid metabolism by oxidizing substrates such as 17β-estradiol, retinol, retinal, and leukotriene B4. Related references: PMID:29562163 PMID:30415504 PMID:32973038
• The protein possesses retinol/retinal dehydrogenase activity in vitro.
• Post-translational modifications include phosphorylation, and alternative splicing generates multiple isoforms.
• As an oxidoreductase, HSD17B13 depends on NAD as a cofactor.

Experimental Guidance and Technical Tips

• For Western blot, anticipate a band at approximately 34 kDa. Validate expression in liver tissue lysates or relevant cell models.
• When performing immunohistochemistry on paraffin-embedded sections, consider optimizing antigen retrieval conditions for lipid droplet-associated proteins.
• For ELISA, pairing with a monoclonal capture/detection antibody may enhance sensitivity; evaluate matrix effects and cross-reactivity.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and appropriate negative controls (e.g., knockdown or knockout samples) are recommended when interpreting results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive, one-stop supply chain for IVD raw materials, supporting diagnostic manufacturers and research laboratories from discovery to clinical application. For your HSD17B13-related projects, we can source validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your hepatic and metabolic disease research. Partner with us to simplify procurement and accelerate your assay development.