Anti-HSD17B13 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q7Z5P4

Core Product Specifications and Parameters

Biological Background: HSD17B13 Function and Localization

• HSD17B13 (17-beta-hydroxysteroid dehydrogenase 13), also known as SCDR9, SDR16C3, FLDP, NIIL497, and HMFN0376, is a member of the short-chain dehydrogenase/reductase (SDR) family.
• It plays a critical role in hepatic lipid metabolism; in vitro, it catalyzes the oxidation of lipid substrates including 17beta-estradiol, retinol, retinal, and leukotriene B4 (PubMed:29562163, PubMed:30415504, PubMed:32973038). Related references: PMID:29562163, PMID:30415504, PMID:32973038
• In vitro enzymatic studies show conflicting results regarding its retinol/retinal dehydrogenase activity.
• The protein localizes to lipid droplets, the endoplasmic reticulum, and the cytoplasm.
• HSD17B13 is predominantly expressed in the liver, with additional expression in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder, and testis (PubMed:29562163). Related references: PMID:29562163
• The gene undergoes alternative splicing, and the protein is subject to phosphorylation; it contains a signal peptide and has an available 3D structure.
• Keywords associated with HSD17B13 include oxidoreductase, NAD, lipid metabolism, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein corresponding to amino acids 34-300 of human HSD17B13 (NP_835236.2), spanning a large portion of the protein. This design may help target conserved epitopes and improve reactivity across species.
• For Western blot (WB), the predicted molecular weight is approximately 34 kDa; use liver tissue lysates as a positive control due to high hepatic expression.
• When performing immunofluorescence (IF-P) or immunohistochemistry (IHC-P), optimize fixation and permeabilization conditions to preserve both cytoplasmic and organelle-localized signals.
• Cross-reactivity with mouse and rat is indicated, making this antibody a potential tool for comparative in vivo studies; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering comprehensive access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. This HSD17B13 Rabbit mAb supports hepatic lipid metabolism research and liver-related diagnostic assay development. With technical services and consulting spanning concept to clinic, CamelBio streamlines the development path for your diagnostic projects.