Anti-HSD17B1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14061

Core Product Specifications and Parameters

Biological Background: HSD17B1 Function and Localization

• HSD17B1 encodes 17-beta-hydroxysteroid dehydrogenase type 1 (17β-HSD1), also known as estradiol 17-beta-dehydrogenase 1 (E2DH) and 20-alpha-hydroxysteroid dehydrogenase (20-alpha-HSD).
• Catalyzes the NADH-dependent reduction of estrone (E1) to the more potent estrogen 17β-estradiol (E2), a critical step in estrogen biosynthesis. Related references: PMID:8994190
• Possesses 20-alpha-HSD activity, converting progesterone to 20α-hydroxyprogesterone, linking it to progestin metabolism.
• Localizes predominantly to the cytoplasm, consistent with its role in steroid metabolism.
• Belongs to the short-chain dehydrogenase/reductase (SDR) family, specifically the SDR28C1 subfamily.
• Participates in lipid and steroid biosynthetic processes, with implications for hormone-dependent tissues.
• Post-translationally modified by phosphorylation, identified through proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human HSD17B1 protein (amino acids 1–328), suggesting the polyclonal antibody may recognize multiple epitopes.
• For Western blot, expect a band at approximately 35 kDa; validate specificity and sensitivity in your relevant sample system.
• IHC‑P and IF/ICC applications may require optimization of antigen retrieval and permeabilization steps for best results.
• High sequence conservation between human, mouse, and rat supports cross-species reactivity; confirm in your experimental models.
• ELISA performance should be separately validated using the specific assay format and detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This HSD17B1 polyclonal antibody is part of our steroid metabolism research collection, supporting diagnostic and life science applications. For custom sourcing of rare targets or integrated consulting from concept to clinic, our team offers personalized assistance and one-stop procurement solutions.