Anti-HSD11B2 Rabbit Polyclonal Antibody for WB, ELISA - P80365

Core Product Specifications and Parameters

Biological Background: HSD11B2 Function and Localization

• HSD11B2 is a microsomal enzyme belonging to the short-chain dehydrogenase/reductase family, catalyzing the NAD-dependent oxidation of active 11beta-hydroxyglucocorticoids (e.g., cortisol) to inactive 11-ketoglucocorticoids (e.g., cortisone). Related references: PMID:10497248 PMID:12788846 PMID:17314322
• By inactivating glucocorticoids, HSD11B2 protects the nonselective mineralocorticoid receptor from inappropriate glucocorticoid occupancy, crucial for electrolyte balance and blood pressure regulation. Related references: PMID:10497248 PMID:12788846 PMID:17314322
• It also oxidizes 11beta-hydroxytestosterone to 11-ketotestosterone, a potent androgen, and converts 11beta-hydroxyandrostenedione to 11-ketoandrostenedione, contributing to androgen metabolism. Related references: PMID:22796344 PMID:27927697 PMID:23685396
• HSD11B2 mediates the conversion of 7-beta-25-dihydroxycholesterol to 7-oxo-25-hydroxycholesterol, which may modulate immune cell migration via the EBI2 receptor. Related references: PMID:30902677
• Localized to the endoplasmic reticulum and microsomal fraction, consistent with its role in steroid metabolism.
• Tissue expression is highest in kidney, placenta, pancreas, prostate, ovary, small intestine, and colon, with lower levels in spleen and testis; at midgestation, it is abundant in placenta and fetal kidney. Related references: PMID:7859916 PMID:8530071
• Defects in HSD11B2 are implicated in apparent mineralocorticoid excess (AME), characterized by hypertension and hypokalemia.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 266–405 of human HSD11B2 (C-terminal region), which may contribute to recognition of epitopes in the catalytic domain.
• For western blot, a band at approximately 44 kDa is anticipated; consider using human kidney or placenta lysates as positive controls based on tissue expression data.
• Cross-reactivity with rat is reported; validate detection in rat samples if required for your experimental system.
• In ELISA applications, the antibody may be used to detect recombinant or endogenous HSD11B2; appropriate coating and detection conditions should be optimized empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to validated IVD raw materials. This HSD11B2 polyclonal antibody supports research into glucocorticoid regulation, mineralocorticoid receptor protection, and androgen metabolism. Beyond individual antibodies, we offer matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development from concept to clinic.