Anti-HSCB Rabbit Polyclonal Antibody for WB and ELISA - Q8IWL3

Core Product Specifications and Parameters

Biological Background: HSCB Function and Localization

• Encoded by HSCB (also known as DNAJC20, HSC20, JAC1, SIDBA5), the iron-sulfur cluster co-chaperone protein HscB belongs to the DnaJ homolog subfamily C member 20.
• Acts as a co-chaperone in mitochondrial iron-sulfur cluster assembly, required for incorporation into SDHB, a complex II subunit (PubMed:20668094; PubMed:26749241). Related references: PMID:20668094 PMID:26749241
• Also functions in cytoplasmic iron-sulfur cluster biogenesis, mediating complex formation with CIA targeting components and insertion into client proteins such as POLD1, ELP3, DPYD, and PPAT (PubMed:29309586). Related references: PMID:29309586
• Essential for hematopoiesis (By similarity).
• Localizes to both cytoplasm and mitochondrion.
• Expressed in multiple tissues including lung, brain, stomach, spleen, ovary, testis, liver, muscle, and heart.
• Keywords from UniProt include chaperone, metal-binding, and proteomics identification.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 27 kDa corresponding to the molecular weight of HSCB; validate with appropriate controls and consider potential post-translational modifications that may shift mobility.
• For ELISA, the antibody can be used as a capture or detection reagent; optimal working dilution should be empirically determined using standard checkerboard titrations.
• Since the immunogen covers amino acids 30–235 (nearly the full-length protein), the antibody may recognize multiple epitopes; verify specificity by peptide competition or using knockout/knockdown lysates when available.
• Store at -20°C upon receipt, avoiding repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This HSCB antibody, targeting a key player in iron-sulfur cluster assembly, exemplifies our commitment to providing high-quality reagents for mitochondrial and metabolic disease research. For custom or rare target sourcing, our global supply network ensures efficient procurement from concept to clinic.