Anti-Hsc70-interacting protein (HIP) Monoclonal Antibody for WB - P50502

Core Product Specifications and Parameters

Biological Background: Hsc70-interacting protein (HIP) Function and Localization

• Hsc70-interacting protein (HIP), also known as ST13, aging-associated protein 2, or progesterone receptor-associated p48 protein, is a 41 kDa cytoplasmic co-chaperone encoded by the ST13 gene.
• It functions as a co-chaperone for the heat shock cognate 71 kDa protein (HSC70), binding to the ATPase domains of at least two HSC70 molecules upon activation by HSP40.
• HIP stabilizes the ADP-bound state of HSC70, which has high affinity for substrate proteins, thereby facilitating the chaperone cycle and protein folding.
• Through its intrinsic chaperone activity, HIP may directly contribute to the interaction of HSC70 with various target proteins, assisting in protein quality control and stress responses.
• Localized predominantly in the cytoplasm, HIP is subject to regulatory post-translational modifications including acetylation and phosphorylation.
• HIP contains tetratricopeptide repeat (TPR) domains that mediate its interaction with HSC70 and other co-chaperones in the multi-chaperone complex.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 270-369 of human HIP (UniProt P50502); consider verifying the antibody’s reactivity with your target species if beyond human and mouse.
• For Western blot, expect a band near 41 kDa under reducing conditions; validate molecular weight standards and loading controls for your specific sample type.
• This antibody is recommended for WB and ELISA; other applications may require individual optimization.
• Consider cross-reactivity with closely related chaperones; validate specificity in the relevant sample context.
• Pair with appropriate secondary antibodies and detection reagents for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated supply solutions for IVD raw materials, supporting diagnostic manufacturers and researchers from early R&D to clinical application. This anti-HIP monoclonal antibody targets chaperone biology and protein homeostasis research, and we can further supply validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to explore how our one-stop platform can streamline your procurement of critical immunoassay components.