Anti-HS3ST3A1 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y663

Core Product Specifications and Parameters

Biological Background: HS3ST3A1 Function and Localization

• HS3ST3A1 encodes for heparan sulfate glucosamine 3-O-sulfotransferase 3A1 (3-OST-3A), a member of the sulfotransferase family that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as a sulfate donor.
• Catalyzes the O-sulfation of glucosamine residues within heparan sulfate, specifically on IdoUA2S-GlcNS and IdoUA2S-GlcNH2 motifs. Related references: PMID:10520990, PMID:9988768
• The 3-O-sulfated heparan sulfate generated by this enzyme serves as a receptor for Herpes simplex virus-1 (HSV-1) entry. Related references: PMID:10520990
• Unlike the related enzyme HS3ST1/3-OST-1, HS3ST3A1 does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate. Related references: PMID:10520990
• Subcellular localization is at the Golgi apparatus membrane, consistent with its role in post-translational modification of heparan sulfate chains.
• Tissue expression is ubiquitous, with highest levels observed in heart and placenta, followed by liver and kidney.
• Post-translational modifications include glycosylation and disulfide bond formation; the protein contains a signal-anchor and transmembrane domain.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 44–140, a region predicted to be extracellular/luminal, likely accessible for antibody recognition in native conformations.
• Predicted molecular weight is 45 kDa; uniprot-calculated weight is 44.9 kDa. Consider running appropriate molecular weight markers and validating with positive controls in WB.
• ELISA can be optimized using recombinant HS3ST3A1 protein or cell lysates from tissues with high expression (heart, placenta).
• Sensitivity and specificity should be confirmed with knockdown or knockout validation in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

As an integrated IVD raw material supplier, CamelBio offers a comprehensive portfolio of antibodies, including this HS3ST3A1 rabbit polyclonal antibody suitable for glycobiology and viral entry mechanism studies. We provide validated antibody pairs, optimized monoclonal and polyclonal reagents, and ancillary bulk materials to streamline diagnostic development from discovery to clinical application.