Anti-HS1BP3 Polyclonal Antibody for WB, ELISA - Q53T59

Core Product Specifications and Parameters

Biological Background: HS1BP3 Function and Localization

• HS1BP3 (HCLS1-binding protein 3), also known as HS1-binding protein 3 (HSP1BP-3) or ETM2, is encoded by the HS1BP3 gene.
• The protein is suggested to be a modulator of IL-2 signaling, a cytokine pathway critical for T-cell proliferation and immune responses.
• HS1BP3 is annotated as an acetylated and phosphorylated protein, indicating that post-translational modifications may regulate its activity or interactions.
• It belongs to the reference proteome for humans and has been identified through high-throughput proteomics, though its detailed subcellular localization remains uncharacterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–200 amino acids of human HS1BP3, which may present epitopes for detecting the native protein across species due to conserved regions.
• For Western blot, validate antibody specificity in relevant human, mouse, or rat cell lines; a band near 43 kDa should be observed, but additional bands may appear due to post-translational modifications or isoforms.
• In ELISA applications, the polyclonal nature may enhance sensitivity; consider optimizing coating and detection conditions with purified HS1BP3 fragments or cell lysates.
• Experimentally confirm the antibody’s performance by knockdown or knockout controls to ensure specific detection of HS1BP3.
• Since HS1BP3 is implicated in IL-2 signaling, perform functional assays under IL-2–stimulated conditions to assess pathway involvement and potential blocking effects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to a comprehensive range of IVD raw materials. For studies involving IL-2 signaling and immune regulation, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. Our integrated capabilities help accelerate your development from bench to clinical application.