Anti-HRPT2/CDC73 Rabbit Monoclonal Antibody for WB and ELISA - Q6P1J9

Core Product Specifications and Parameters

Biological Background: Parafibromin Function and Localization

• Parafibromin, also known as cell division cycle protein 73 homolog (CDC73) or hyperparathyroidism 2 protein, is a tumor suppressor encoded by the CDC73 gene (also C1orf28, HRPT2).
• It is a key component of the RNA polymerase II-associated factor 1 (PAF1) complex (PAF1C), which regulates transcriptional elongation, histone modifications, and mRNA processing.
• PAF1C is involved in histone H2B monoubiquitination at Lys-120 (H2BK120ub1) and histone H3 Lys-4 trimethylation (H3K4me3), coupling transcription with chromatin modifications.
• The complex associates with RNA polymerase II via interactions with the C-terminal domain (CTD) and is required for the expression of Hox and Wnt target genes, linking it to developmental pathways.
• Parafibromin connects the PAF1 complex with cleavage and polyadenylation factors, participating in mRNA 3' end formation and polyadenylation.
• Subcellularly, parafibromin localizes to the nucleus, consistent with its roles in transcription and RNA processing.
• It is expressed in adrenal and parathyroid glands, kidney, and heart, and its dysfunction is associated with hyperparathyroidism-jaw tumor syndrome.
• Post-translational modifications include phosphorylation and acetylation, and it is implicated in cell cycle progression through regulation of cyclin D1 expression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300-400 of human CDC73, which may target the C-terminal region; users should consider this when designing domain-specific detection experiments.
• For Western blot, a band at approximately 61 kDa is expected; validate detection in human, mouse, and rat cell lysates due to reported cross-reactivity.
• In ELISA applications, consider using the recombinant protein as a positive control; optimal antibody dilution should be determined experimentally.
• Since parafibromin functions in transcriptional complexes, consider nuclear fraction enrichment or co-immunoprecipitation assays to study its interactions.

CamelBio: Your One-Stop Sourcing Bridge

This anti-HRPT2/CDC73 rabbit monoclonal antibody supports research into transcriptional regulation and tumor suppression. CamelBio empowers diagnostic manufacturers and labs with a comprehensive supply chain—from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents and custom sourcing for rare targets. Explore our integrated raw-material solutions to streamline your diagnostic development.