Anti-HRPT2 / CDC73 Rabbit Polyclonal Antibody for WB and ELISA - Q6P1J9

Core Product Specifications and Parameters

Biological Background: Parafibromin (HRPT2/CDC73) Function and Localization

• Parafibromin, also known as cell division cycle protein 73 homolog or hyperparathyroidism 2 protein, is a tumor suppressor likely involved in transcriptional and post-transcriptional control pathways. It may regulate cell cycle progression through modulation of cyclin D1/PRAD1 expression.
• As a core component of the PAF1 complex (PAF1C), parafibromin associates with RNA polymerase II to promote transcriptional elongation, histone modifications (such as H2B monoubiquitination and H3K4 trimethylation), and mRNA 3' end formation and polyadenylation.
• PAF1C is critically required for transcription of Hox genes and Wnt target genes, thereby linking parafibromin to Wnt signaling and embryonic development.
• In hematopoiesis, PAF1C stimulates KMT2A/MLL1 transcriptional activity and cooperates with MLL-rearranged oncoproteins (e.g., MLL-AF9, MLL-ENL) to promote leukemogenesis.
• Parafibromin localizes predominantly to the nucleus.
• Tissue specificity has been reported in adrenal and parathyroid glands, kidney, and heart.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), consistent with its roles in signaling and protein stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 435–505 at the C-terminus of human parafibromin, targeting a region that may yield high specificity for full-length protein detection by Western blot.
• For Western blotting, validate antibody performance in human cell lines or tissues with known CDC73 expression, such as parathyroid or kidney lysates, and confirm detection of the expected 61 kDa band.
• When performing ELISA, consider pairing this antibody as a capture or detection reagent with a matched antibody pair; titrate to determine optimal working concentrations for your specific assay format.
• As a polyclonal antibody, it may recognize multiple epitopes; verify linearity and sensitivity in your sample matrix before quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing solutions for IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For researchers focused on tumor suppressors and transcriptional regulation, we provide reliable access to reagents targeting parafibromin and related pathway components. Our expertise streamlines the transition from concept to clinic, ensuring efficient procurement and technical support for your diagnostic development needs.