Anti-HRAS Polyclonal Antibody for WB, IHC-P, ELISA - P01112

Core Product Specifications and Parameters

Biological Background: HRAS Function and Localization

• HRAS encodes the GTPase HRas, also known as H-Ras-1, Ha-Ras, transforming protein p21, c-H-ras, and p21ras.
• It functions as a signal transducer in the Ras-MAPK signaling pathway, playing a critical role in the regulation of cell proliferation and survival. Related references: PMID:22821884
• Ras proteins bind GDP/GTP and possess intrinsic GTPase activity, allowing them to act as molecular switches. Related references: PMID:12740440, PMID:14500341, PMID:9020151
• HRas is ubiquitinated by the LZTR1-BCR E3 ubiquitin ligase complex, which regulates its stability and signaling output. Related references: PMID:40934300
• The protein localizes to multiple cellular compartments including the cell membrane, Golgi apparatus membrane, nucleus, cytoplasm, and perinuclear region.
• It is widely expressed across human tissues.
• Post-translational modifications such as palmitoylation, prenylation, acetylation, methylation, and ubiquitination modulate its localization and activity.
• HRAS is a proto-oncogene, and mutations in this gene are associated with various cancers and developmental disorders (RASopathies).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the N-terminal region (amino acids 1-100) of human HRAS. Sequence conservation supports expected cross-reactivity with mouse and rat, as indicated.
• For Western blot (WB), expect a band near 21 kDa. Include appropriate positive controls (e.g., lysates from human, mouse, or rat cell lines) and validate in your sample system.
• In immunohistochemistry (IHC-P), consider optimizing antigen retrieval and validate staining specificity using known positive and negative tissues.
• For ELISA, optimize coating antigen and detection conditions. Peptide competition or positive control antibodies may assist in assay development.
• As a polyclonal antibody, lot-to-lot consistency should be validated, particularly for quantitative or regulated applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. For HRAS-related projects, we supply validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing of rare target raw materials—helping to accelerate your assay development and scalability from concept to clinic.