Anti-HRAS Polyclonal Antibody for WB, IF/ICC, ELISA - P01112

Core Product Specifications and Parameters

Biological Background: HRAS Function and Localization

• GTPase HRas, also known as H-Ras-1, Ha-Ras, transforming protein p21, c-H-ras, and p21ras, is encoded by the HRAS gene (also known as HRAS1) in humans.
• Serves as a signal transducer in the Ras-MAPK signaling pathway, regulating cell proliferation and survival. Related references: PMID:22821884
• Binds GDP/GTP and possesses intrinsic GTPase activity, cycling between an inactive GDP-bound state and an active GTP-bound state. Related references: PMID:12740440 PMID:14500341 PMID:9020151
• Recognized by LZTR1, which mediates its ubiquitination by a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. Related references: PMID:40934300
• Localizes to the cell membrane, Golgi apparatus membrane, nucleus, cytoplasm, and perinuclear region, consistent with its role in multiple signaling compartments.
• Classified as a proto-oncogene; activating mutations are frequent in various cancers, making it a key target in oncology research.
• Post-translational modifications include acetylation, lipidation (palmitoylation and prenylation), methylation, S-nitrosylation, and ubiquitin conjugation, which regulate its membrane association and activity.
• Widely expressed across human tissues, underscoring its fundamental role in cell growth and differentiation.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length protein (amino acids 1–189), so the antibody may recognize both native and denatured conformations, supporting its validated use in WB, IF/ICC, and ELISA.
• For WB, the predicted band is approximately 21 kDa; consider using human cancer cell lines (e.g., HeLa or A431) known to express HRAS as positive controls, and validate the band size with appropriate markers.
• In IF/ICC, fixation and permeabilization conditions may need optimization; begin with paraformaldehyde fixation followed by Triton X-100 treatment, as HRAS is membrane- and cytoplasm-localized.
• For ELISA, the antibody can function as either a capture or detection reagent; however, pairing with a matched antibody specific to a different epitope is recommended for sandwich formats.
• Because the antibody cross-reacts with mouse HRAS, it can be employed in mouse model studies; always include species-appropriate controls to confirm reactivity.
• The product is shipped on ice bags; upon receipt, store at -20°C or as recommended to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs by providing validated IVD raw materials, including this anti-HRAS polyclonal antibody for signaling and oncology-focused assay development. We offer a comprehensive portfolio of antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and specialized rare-target sourcing, enabling efficient transition from concept to clinic. Partner with us to streamline your Ras-MAPK pathway research and diagnostic kit production.