Anti-HR Polyclonal Antibody for WB and ELISA - O43593

Core Product Specifications and Parameters

Biological Background: HR Function and Localization

• HR (Hairless) is a lysine-specific histone demethylase, also known by aliases AU, MUHH, ALUNC, HYPT4, MUHH1, and HSA277165, encoded by the HR gene.
• It specifically demethylates mono- and dimethylated lysine-9 of histone H3 (H3K9me1/me2), thereby regulating chromatin structure and gene transcription.
• HR functions as a transcription regulator critical for hair biology, likely through the targeting of collagen genes, and also modulates neural activity and cell cycle progression.
• The protein is localized to the nucleus, consistent with its role in histone modification and transcription.
• Isoform 1 and isoform 2 show highest expression in the small intestine; isoform 2 is strongly expressed in skin, while isoform 1 is weakly expressed in brain and colon, with trace amounts in other tissues.
• HR contains zinc-finger domains and relies on iron as a cofactor for its demethylase activity, placing it in the JmjC domain-containing demethylase family.
• Post-translational modifications and alternative splicing generate multiple isoforms; mutations in HR are associated with the hypotrichosis disease phenotype.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 200–480 of human HR, which may generate antibodies that recognize multiple HR isoforms if the epitope sequences are conserved.
• For Western blotting, the predicted molecular weight of HR is ~127 kDa; use a standard SDS-PAGE gel with appropriate acrylamide percentage (e.g., 8–10%) and standard wet or semi-dry transfer protocols for high molecular weight proteins.
• For ELISA applications, optimize the coating concentration of the antigen and use a blocking step with non-fat milk or BSA to reduce background.
• As HR is expressed in skin and small intestine, these tissues can serve as positive controls; validate the antibody specificity by including lysates from HR-expressing cell lines or tissues, and consider using knockout cell lines if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material sourcing, offering a wide range of validated antibodies, including this anti-HR polyclonal antibody, to support research into histone modification and hair biology. We provide one-stop access to antibody pairs, bulk ancillary reagents, and customized rare target sourcing, empowering diagnostic manufacturers and researchers to accelerate their projects from concept to clinic.