Anti-HPRT1 Polyclonal Antibody for WB, ELISA - P00492

Core Product Specifications and Parameters

Biological Background: HPRT1 Function and Localization

• HPRT1 (also known as HPRT or HGPRT) is a purine salvage enzyme that catalyzes the conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate, using 5-phosphoribosylpyrophosphate as a phosphoribosyl donor.
• This enzyme is central to the purine salvage pathway, recycling preformed purine bases to maintain intracellular nucleotide pools.
• HPRT1 is primarily localized in the cytoplasm, consistent with its metabolic role in nucleotide synthesis.
• It belongs to the glycosyltransferase family, requiring magnesium as a cofactor for its transferase activity.
• Post-translational modifications, including acetylation and phosphorylation, may modulate its function.
• Mutations in HPRT1 are linked to Lesch-Nyhan syndrome, a severe neurological disorder, and gout, highlighting its clinical importance.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length HPRT1 protein (residues 1-218), likely generating antibodies that recognize both native and denatured forms, suitable for Western blotting.
• For ELISA, consider using the recombinant immunogen as a coating antigen to capture specific antibodies.
• Cross-reactivity with mouse and rat suggests conserved epitopes; validate performance in the relevant sample systems.
• Under standard reducing conditions, HPRT1 migrates at approximately 25 kDa in SDS-PAGE; optimize transfer and blocking conditions for WB detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, offering researchers and diagnostic developers streamlined access to a wide portfolio of antibodies, including this anti-HPRT1 rabbit polyclonal antibody for purine metabolism studies. Whether you need validated antibody pairs, bulk ancillary reagents, or rare-target raw materials, our one-stop supply chain supports your projects from early research to clinical application.