Anti-HPD Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P32754

Core Product Specifications and Parameters

Biological Background: HPD Function and Localization

• HPD catalyzes the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, a critical step in tyrosine catabolism.
  Related references: PMID:10942115, PMID:1339442, PMID:34047349
• HPD may also function as an N6-adenosine-methyltransferase, methylating adenosine residues at the N(6) position of certain RNAs.
  Related references: PMID:10942115
• Alternative splicing generates multiple transcript variants encoding different isoforms.
• The protein localizes to the cytoplasm, endoplasmic reticulum membrane, Golgi apparatus membrane, and nucleus.
• Post-translational modifications include acetylation and phosphorylation.
• Contains an iron-binding active site; mutations in HPD are associated with tyrosinemia type III, characterized by intellectual disability.
• Keywords: Acetylation, Alternative splicing, Cytoplasm, Dioxygenase, Disease variant, Endoplasmic reticulum, Golgi apparatus, Intellectual disability, Iron, Membrane, Metal-binding, Nucleus, Oxidoreductase, Phenylalanine catabolism, Phosphoprotein, Transferase, Tyrosine catabolism.

Experimental Guidance and Technical Tips

• For Western blotting, a dominant band around 45 kDa is expected; confirm specificity with knockdown or knockout controls.
• For immunohistochemistry (IHC-P), consider antigen retrieval and validate with tissue known to express HPD.
• ELISA applications may utilize this polyclonal antibody as a capture or detection reagent; pair with a monoclonal antibody for enhanced specificity.
• Cross-reactivity with mouse and rat has been reported; verify sequence alignment and expression patterns in target tissues.
• As a rabbit polyclonal antibody, it recognizes multiple epitopes; pre-adsorption may be considered for highly specific assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-HPD antibody supports research into tyrosine metabolism and metabolic disorders. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your IVD development.