Anti-HPCAL1 Rabbit Polyclonal Antibody for WB, IP, ELISA - P37235

Core Product Specifications and Parameters

Biological Background: HPCAL1 Function and Localization

• HPCAL1 (Hippocalcin-like protein 1), also known as BDR-1, HLP2, or Visinin-like protein 3 (VILIP-3), is a calcium-binding protein belonging to the neuronal calcium sensor family.
• The protein contains EF-hand domains that mediate calcium binding, as indicated by its classification under the keywords 'Calcium' and 'Metal-binding'.
• HPCAL1 is predicted to associate with cellular membranes, consistent with its myristoylation site and the keywords 'Lipoprotein' and 'Myristate'.
• It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation, suggesting a potential role in photoreceptor function or phototransduction.
• The protein has a theoretical molecular weight of approximately 22.3 kDa; post-translational modifications such as myristoylation can influence its membrane affinity and localization.
• Structural studies (keyword '3D-structure') have provided insights into the conformational changes induced by calcium binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human HPCAL1 protein; the polyclonal antibody is expected to recognize native and denatured forms, making it suitable for multiple applications.
• For Western blot, a band near 22 kDa is anticipated; consider using a denaturing gel and transfer to nitrocellulose or PVDF membranes.
• In immunoprecipitation, pre-clearing and optimization of antibody-to-lysate ratios are recommended; validate in the relevant human, mouse, or rat tissue or cell line.
• For ELISA, the antibody can be used as a capture or detection reagent; appropriate blocking and washing conditions should be optimized for each assay format.
• Because this is a polyclonal antibody, batch-to-batch consistency should be verified; consider aliquoting and storing at -20°C after reconstitution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with comprehensive access to high-quality raw materials, from validated antibodies to bulk reagents. For calcium signaling and neuroscience targets like HPCAL1, we offer ready-to-use polyclonal antibodies and can assist in sourcing matched antibody pairs, recombinant proteins, and ancillary reagents. Contact us to simplify your supply chain and accelerate development with reliable, cost-effective solutions.