Anti-HP1BP3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q5SSJ5

Core Product Specifications and Parameters

Biological Background: HP1BP3 Function and Localization

• HP1BP3 (Heterochromatin protein 1-binding protein 3), also known as HP1-BP74, is a component of heterochromatin that maintains heterochromatin integrity during G1/S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity. Related references: PMID:24830416
• It mediates chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance and self-renewal. Related references: PMID:25100860
• HP1BP3 localizes to the nucleus and chromosomes, consistent with its role in chromatin organization.
• The protein contains DNA-binding domains and repeats, and undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination (Ubl conjugation).
• Alternative splicing generates multiple isoforms; the full-length protein has a predicted molecular weight of approximately 61 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-160) of human HP1BP3, which is expected to recognize full-length HP1BP3 and possibly isoforms sharing this region. Consider sequence alignment if cross-reactivity with other HP1-binding proteins is a concern.
• For Western blot, the predicted molecular weight is ~61 kDa. Use a validated positive control lysate (e.g., HeLa or HEK293) and confirm specificity with siRNA knockdown or blocking peptide if needed.
• For IHC-P, test different antigen retrieval methods (e.g., citrate buffer pH 6.0) and antibody dilutions; validate in tissue samples known to express HP1BP3, such as tumor tissues.
• In ELISA, the polyclonal antibody can be used as a capture or detection reagent; for quantitative assays, consider optimizing the coating buffer and pairing with a matched monoclonal antibody.
• The antibody is reactive with human, mouse, and rat; however, performance may vary across species and applications, so validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials and technical services. In the chromatin biology and cell cycle research field, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic. Explore our HP1BP3-related reagents to speed your diagnostic workflows.