Anti-HP1 gamma/CBX3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q13185

Core Product Specifications and Parameters

Biological Background: HP1 gamma/CBX3 Function and Localization

• Chromobox protein homolog 3, also known as HP1 gamma, HECH, or modifier 2 protein, is a conserved heterochromatin component encoded by the CBX3 gene.
• It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Related references: PMID:11242053
• It also binds histone H1.4 methylated at Lys-26, but is excluded from chromatin when H1.4 is simultaneously methylated at Lys-26 and phosphorylated at Ser-27. Related references: PMID:16127177
• HP1 gamma is involved in the formation of functional kinetochores through interaction with the MIS12 complex. Related references: PMID:15502821
• It contributes to the conversion of local chromatin to a heterochromatin-like repressive state via H3 Lys-9 trimethylation and mediates the recruitment of SUV39H1 and/or SUV39H2 by the PER complex to circadian target genes.
• The protein recruits NIPBL to sites of DNA double-strand breaks, playing a role in DNA damage response. Related references: PMID:28167679
• Subcellular localization is predominantly nuclear, consistent with its chromatin-associated functions.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, which likely regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-183 of human HP1 gamma, which encompasses the chromodomain and part of the chromoshadow domain; this design targets both functional domains.
• For Western blot (WB), the predicted molecular weight is 21 kDa; validate detection in your sample type and consider using nuclear extracts for enrichment.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions to ensure epitope accessibility.
• The antibody is suitable for immunoprecipitation (IP) and ELISA; when using IP, confirm pull-down efficiency with appropriate controls.
• Cross-reactivity is reported for human, mouse, and rat; if using other species, verify sequence homology before testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, including this anti-HP1 gamma/CBX3 antibody for epigenetic and chromatin biology applications. Our comprehensive portfolio covers optimized monoclonal and polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents, supporting projects from early-stage development to routine production. For rare or custom targets, our sourcing capabilities help secure high-performance raw materials to accelerate your assay and clinical workflows.