Anti-HP1 gamma/CBX3 Monoclonal Antibody for WB, IHC-P, ELISA - Q13185

Core Product Specifications and Parameters

Biological Background: HP1 gamma/CBX3 Function and Localization

• HP1 gamma/CBX3 (Chromobox protein homolog 3), also known as HECH or Modifier 2 protein, is encoded by the CBX3 gene and belongs to the heterochromatin protein 1 family.
• Recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. Related references: PMID:11242053
• Also recognizes histone H1.4 methylated at 'Lys-26' (H1.4K26me), but is excluded from chromatin when H1.4K26me co-occurs with phosphorylation at Ser-27. Related references: PMID:16127177
• Involved in kinetochore formation via interaction with MIS12 complex proteins. Related references: PMID:15502821
• Contributes to circadian clock regulation by recruiting SUV39H1/2 methyltransferases to E-box elements of circadian target genes (By similarity).
• Mediates recruitment of NIPBL to DNA double-strand breaks, facilitating damage response. Related references: PMID:28167679
• Localizes to the nucleus, consistent with its chromatin-associated functions.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bonds, and ubiquitin-like conjugation, reflecting dynamic regulatory control.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human HP1 gamma/CBX3, a region that may be conserved; verify sequence homology if using with mouse or rat samples to anticipate cross-reactivity.
• For western blot (WB), the predicted molecular weight is ~21 kDa; use appropriate gel percentage (e.g., 10-12% SDS-PAGE) and include molecular weight markers and loading controls.
• In IHC-P, standard antigen retrieval and blocking steps should be optimized for the tissue of interest; consider using a known positive control tissue.
• For ELISA, the antibody may be used as capture or detection reagent; titration and assay conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio of validated antibody pairs, optimized monoclonal antibodies, recombinant proteins, and ancillary reagents. This anti-HP1 gamma/CBX3 rabbit monoclonal antibody, ideal for chromatin and epigenetic research, exemplifies our commitment to providing high-quality reagents. Whether you need bulk quantities, custom antibody development, or rare target sourcing, CamelBio supports your journey from concept to clinic.