Anti-HP1 alpha/CBX5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA, ChIP - P45973

Core Product Specifications and Parameters

Biological Background: HP1 alpha/CBX5 Function and Localization

• HP1 alpha/CBX5 (Chromobox protein homolog 5), also known as Antigen p25 or HP1 alpha, is a conserved heterochromatin protein encoded by the CBX5 gene.
• It binds histone H3 tails dimethylated or trimethylated at Lys-9 (H3K9me), a hallmark of constitutive heterochromatin, thereby mediating epigenetic gene silencing. Related references: PMID:40440427
• Phosphorylation of histone H3 at Tyr-41 (H3Y41ph) displaces HP1 alpha from chromatin, providing a regulatory switch for heterochromatin dynamics. Related references: PMID:19783980
• Recognizes methylated histone H1.4 at Lys-26 (H1.4K26me); simultaneous phosphorylation at Ser-27 (H1.4S27ph) excludes HP1 alpha binding. Related references: PMID:16127177
• Interacts with lamin-B receptor (LBR) to tether heterochromatin to the inner nuclear membrane, contributing to nuclear architecture. Related references: PMID:19783980
• Essential for proper kinetochore and inner centromere assembly via interaction with the MIS12 complex subunit NSL1, crucial for chromosome segregation. Related references: PMID:19783980 PMID:20231385
• Localizes predominantly to the nucleus and accumulates at centromeric heterochromatin and chromosome arms.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation; also implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-191 amino acids of human HP1 alpha, suggesting the antibody may recognize full-length HP1 alpha and possibly isoforms containing this region.
• For Western blot, the predicted molecular weight is ~22 kDa, but post-translational modifications may affect electrophoretic migration; consider loading appropriate controls.
• For chromatin immunoprecipitation (ChIP), the antibody's capacity to bind H3K9me3-associated HP1 alpha makes it suitable for heterochromatin analysis; validate antibody specificity in the relevant sample system.
• Optimize fixation and antigen retrieval conditions for IHC-P and IF/ICC, as epitope accessibility may vary depending on sample preparation.
• Cross-reactivity with mouse and rat HP1 alpha is indicated, but verify performance if using non-human samples in downstream assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and technical services to support diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-HP1 alpha/CBX5 rabbit polyclonal antibody exemplifies our commitment to providing high-quality reagents for chromatin and epigenetic studies. From validated antibody pairs to rare-target raw-material sourcing, CamelBio delivers one-stop solutions to accelerate your assay development and production needs.