Anti-HP1 alpha/CBX5 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P45973

Core Product Specifications and Parameters

Biological Background: HP1 alpha/CBX5 Function and Localization

• HP1 alpha is a component of heterochromatin that specifically recognizes and binds histone H3 tails methylated at Lys-9 (H3K9me), leading to epigenetic repression. Related references: PMID:40440427
• Binding to H3K9me is excluded when Tyr-41 of histone H3 is phosphorylated (H3Y41ph). Related references: PMID:19783980
• Also recognizes histone H1.4 methylated at Lys-26 (H1.4K26me), but this binding is disrupted when H1.4 is simultaneously phosphorylated at Ser-27 (H1.4S27Ph). Related references: PMID:16127177
• HP1 alpha may contribute to heterochromatin association with the inner nuclear membrane via interactions with lamin-B receptor (LBR). Related references: PMID:19783980
• Involved in kinetochore formation through interaction with the MIS12 complex subunit NSL1, and required for inner centromere formation. Related references: PMID:19783980, PMID:20231385
• Subcellular localization includes nucleus, chromosomes, and specifically centromeres, reflecting its role in chromatin organization.
• Keywords indicate post-translational modifications: acetylation and phosphorylation, as well as potential ubiquitination (Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 92-191) lies within the chromo shadow domain of HP1 alpha; researchers should verify epitope accessibility in their assay context.
• For Western blot, the predicted molecular weight is 22 kDa; consider using appropriate gel percentage and transfer conditions for small proteins.
• This antibody is validated for multiple applications (WB, IHC-P, IF/ICC, IP, ELISA) but optimal dilutions should be determined empirically in each laboratory.
• Cross-reactivity is reported for human, mouse, and rat; validate target specificity in other species if required.
• When performing chromatin immunoprecipitation (ChIP) or similar assays, note that HP1 alpha binding is methylation-dependent; include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For chromatin biology and epigenetic targets like HP1 alpha/CBX5, our sourcing services cover rare and custom raw materials to support your diagnostic development pipeline from concept to clinic. Contact us for tailored procurement solutions.