Anti-HP1 alpha/CBX5 Polyclonal Antibody for WB, IF/ICC, ELISA - P45973

Core Product Specifications and Parameters

Biological Background: HP1 alpha/CBX5 Function and Localization

• HP1 alpha (CBX5) is a core component of heterochromatin that recognizes and binds histone H3 tails methylated at Lys-9 (H3K9me), leading to epigenetic repression. Related references: PMID:40440427
• It is excluded from chromatin when histone H3 is phosphorylated at Tyr-41 (H3Y41ph), modulating its repressive activity. Related references: PMID:19783980
• HP1 alpha also interacts with histone H1.4 methylated at Lys-26 (H1.4K26me), but binding is disrupted by simultaneous phosphorylation at Ser-27 (H1.4S27Ph). Related references: PMID:16127177
• It may facilitate the association of heterochromatin with the inner nuclear membrane through interaction with lamin-B receptor (LBR). Related references: PMID:19783980
• HP1 alpha is essential for kinetochore assembly and inner centromere formation, acting via the MIS12 complex subunit NSL1. Related references: PMID:19783980, PMID:20231385
• Subcellularly, it localizes to the nucleus, with enrichment at chromosomes and centromeres.
• Post-translational modifications of HP1 alpha include acetylation, phosphorylation, and ubiquitination, which may regulate its chromatin association and function.
• The protein has a predicted molecular weight of 22.2 kDa, consistent with the observed band at ~22 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 100 amino acids of human HP1 alpha; consider sequence alignment to assess epitope conservation across species and isoforms.
• For Western blot, a band is expected at approximately 22 kDa; use standard SDS‑PAGE and transfer conditions, optimizing antibody dilution for your sample.
• In immunofluorescence/ICC, anticipate nuclear staining with centromeric foci; validate localization using appropriate counterstains and controls.
• For ELISA, titrate the coating antigen and detection antibody to establish optimal signal-to-noise ratios.
• Cross-reactivity has been demonstrated for human and mouse samples; for other species, perform in silico prediction and verify experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories with a comprehensive portfolio of IVD raw materials, exemplified by this anti‑HP1 alpha polyclonal antibody for epigenetics and chromatin studies. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and custom sourcing of rare targets, we offer a streamlined procurement pathway. Partner with us to accelerate your assay development from concept to clinic.