Anti-HOXD9 Polyclonal Antibody for WB, ELISA - P28356

Core Product Specifications and Parameters

Biological Background: HOXD9 Function and Localization

• HOXD9 is a member of the homeobox family of transcription factors, characterized by a conserved homeodomain that facilitates sequence-specific DNA binding.
• It functions within a developmental regulatory system that provides cells with specific positional identities along the anterior-posterior axis, essential for proper embryonic patterning.
• The protein predominantly localizes to the nucleus, where it regulates the transcription of target genes involved in developmental processes.
• Post-translational modifications, particularly phosphorylation, have been identified through proteomics studies, suggesting a layer of regulatory control over its activity.
• HOXD9 is part of the HOXD gene cluster and is also known by several aliases, including HOX4, HOX4C, Hox-4.3, and Hox-5.2.
• UniProt annotates HOXD9 with keywords: Developmental protein, DNA-binding, Homeobox, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transcription, and Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100-200 of human HOXD9; applications may benefit from peptide blocking assays to confirm specificity.
• For Western blot, a band around 36 kDa is expected based on the calculated molecular weight; consider running pre-stained markers and validating in a relevant human sample system.
• ELISA protocols can be developed using this polyclonal antibody, but optimal coating and detection conditions should be empirically determined.
• Cross-reactivity is confirmed for human; reactivity with other species has not been tested, so validate in non-human samples if needed.
• As a polyclonal antibody, batch-to-batch consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials for diagnostic manufacturers and research laboratories, covering every stage from concept to clinic. This anti-HOXD9 polyclonal antibody serves as a valuable tool for studies in developmental biology, gene regulation, and chromatin-linked diseases. We also supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to support your integrated IVD development pipeline.