Anti-HOXD8 Polyclonal Antibody for WB, ELISA - P13378

Core Product Specifications and Parameters

Biological Background: Homeobox protein Hox-D8 Function and Localization

• HOXD8 (Homeobox protein Hox-D8) is a sequence-specific transcription factor involved in developmental regulation.
• It provides cells with specific positional identities on the anterior-posterior axis during embryogenesis.
• The protein features a homeobox DNA-binding domain, which allows it to regulate transcription of target genes.
• Localized exclusively in the nucleus, HOXD8 carries out its transcriptional regulatory functions.
• The HOXD8 gene undergoes alternative splicing, yielding multiple protein isoforms with potential functional diversity.
• Post-translational modifications and proteomics identification have been noted, though specific modifications are not detailed here.
• As a member of the homeobox family, HOXD8 plays a critical role in cell differentiation and morphology.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide from the C-terminal region (aa 200–C-terminus) of human HOXD8. This region is highly conserved, which predicts reactivity across human, mouse, and rat.
• For Western blot, a band at ~32 kDa is expected (based on the provided protein weight). Consider using appropriate loading controls and validating in the relevant sample system.
• The antibody is suitable for ELISA; confirm optimal coating concentration and detection conditions experimentally.
• Due to the nuclear localization of HOXD8, cell fractionation may enhance detection in Western blot assays.
• HOXD8 expression may be developmentally regulated; consider testing multiple tissue types or developmental stages for biological relevance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-HOXD8 polyclonal antibody supports research in developmental biology and gene regulation. Beyond this product, CamelBio supplies validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, ensuring a reliable procurement experience for your diagnostic development needs.