Anti-HOXD10 Rabbit Monoclonal Antibody for WB, ELISA - P28358

Core Product Specifications and Parameters

Biological Background: HOXD10 Function and Localization

• HOXD10 is a homeobox-containing transcription factor belonging to the HOX family, also known as HOX4D, HOX4E, and Hox-4.4.
• It functions as a sequence-specific transcription factor that provides cells with positional identities along the anterior-posterior axis during development.
• Localized to the nucleus, HOXD10 binds DNA and regulates transcription of target genes involved in developmental processes.
• The protein is strongly expressed in the adult male and female urogenital tracts, suggesting a role in urogenital system development and function.
• HOXD10 is annotated as a phosphoprotein, indicating potential regulation by phosphorylation.
• Keywords: Developmental protein, DNA-binding, Homeobox, Nucleus, Phosphoprotein, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-150), so the antibody may recognize both full-length and truncated HOXD10 variants.
• For Western blot, consider using lysates from human and mouse urogenital tissues or cell lines expressing HOXD10; the predicted band is ~38 kDa.
• In ELISA, pair with a suitable detection antibody and validate in the relevant sample system to ensure sensitivity and specificity.
• Cross-reactivity with mouse allows comparative studies; verify reactivity in your specific mouse model before large-scale experiments.
• Titrate the antibody to determine optimal working concentrations for both WB and ELISA, as performance may vary across sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with convenient one-stop access to high-quality IVD raw materials, including validated monoclonal and polyclonal antibodies for developmental biology targets like HOXD10. Beyond this antibody, we offer optimized antibody pairs, bulk ancillary reagents, and custom sourcing support for rare targets, helping streamline your workflow from concept to clinic.