Anti-HOXC4 Polyclonal Antibody for WB, ELISA - P09017

Core Product Specifications and Parameters

Biological Background: HOXC4 Function and Localization

• HOXC4 is a member of the Hox family of homeobox transcription factors, designated as Homeobox protein Hox-C4, also known as CP19 or Hox-3E.
• It functions as a sequence-specific transcription factor that provides cells with specific positional identities along the anterior-posterior axis during development.
• HOXC4 is a developmental regulatory protein involved in establishing body plan morphogenesis.
• The protein contains a homeobox DNA-binding domain, characteristic of the Hox family, enabling it to regulate transcription of target genes.
• Subcellularly, HOXC4 localizes to the nucleus, consistent with its role as a transcription factor.
• Based on UniProt keywords, HOXC4 is associated with developmental processes, DNA-binding, transcription regulation, and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans residues 30–130 of human HOXC4, located in the N-terminal region. This may target epitopes outside the homeodomain, potentially reducing cross-reactivity with other Hox proteins.
• For Western blotting, the expected band size is approximately 30 kDa. Use molecular weight markers and validate with a positive control lysate.
• This antibody is reported to cross-react with mouse and rat HOXC4. However, users should verify reactivity in their sample type before routine use.
• ELISA application may be used for quantification, but optimal antibody concentration and coating conditions should be determined empirically.
• Due to the rabbit polyclonal nature, lot-to-lot variability may occur. Consistent QC measures are employed, but we recommend including internal reference standards in each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio integrates sourcing, technical services, and consulting to provide a one-stop solution for IVD manufacturers and research laboratories. Our portfolio covers a broad range of raw materials, from monoclonal/polyclonal antibodies to recombinant proteins. For developmental biology targets such as HOXC4, CamelBio facilitates access to essential reagents and custom sourcing needs.