Anti-HOXB6 Polyclonal Antibody for WB, ELISA - P17509

Core Product Specifications and Parameters

Biological Background: HOXB6 Function and Localization

• HOXB6 (also known as HOX2B, Hox-2.2, HU-2) is a homeobox protein belonging to the HOX family of transcription factors.
• It functions as a sequence-specific transcription factor that contributes to providing cells with specific positional identities along the anterior-posterior body axis during development.
• HOXB6 localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• The protein is subject to alternative splicing, generating multiple isoforms with potentially distinct regulatory functions.
• HOXB6 contains a conserved homeobox domain that mediates DNA binding and transcriptional control.
• Post-translational modifications include phosphorylation, which may modulate its activity, stability, or interactions.
• As a developmental protein, HOXB6 is implicated in patterning and differentiation processes.

Experimental Guidance and Technical Tips

• The immunogen covers an N-terminal region (aa 30-110) of HOXB6, which may be less conserved among HOX family members; consider verifying specificity in your system by using appropriate control lysates or peptide competition.
• For Western blot, a band of approximately 25 kDa is expected; optimize antibody dilution empirically based on target abundance.
• This antibody has been shown to detect HOXB6 in human and mouse samples; it may also react with other species due to sequence conservation; validate cross-reactivity experimentally if needed.
• ELISA can be used for quantifying HOXB6 expression; ensure coating conditions and detection antibodies are optimized for your assay format.
• As a polyclonal antibody, lot-to-lot variability may occur; consider reserving a test aliquot for consistency checks.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supporting diagnostic manufacturers with a comprehensive portfolio from research to production. This anti-HOXB6 polyclonal antibody is part of our extensive offering for developmental biology and transcription regulation targets. We also supply matched antibody pairs, bulk ancillary reagents, and can source rare-target antibodies to streamline your assay development.