Anti-HOXB2 Rabbit Polyclonal Antibody for WB and ELISA - P14652

Core Product Specifications and Parameters

Biological Background: HOXB2 Function and Localization

• Homeobox protein Hox-B2 (also known as Hox-2.8, Hox-2H, K8) is a sequence-specific transcription factor encoded by the HOXB2 gene in humans.
• It plays a critical role in embryonic development by providing cells with specific positional identities along the anterior-posterior body axis.
• HOXB2 belongs to the Antennapedia-type homeobox gene family and is located within the HOXB cluster on chromosome 17.
• The protein localizes predominantly to the nucleus, where it binds DNA to regulate target gene expression.
• UniProt annotations classify HOXB2 as a phosphoprotein and a developmental protein, with a homeobox DNA-binding domain.
• Conserved across vertebrates, HOXB2 orthologs in mouse and rat share high sequence similarity, as indicated by the antibody’s cross-reactivity.

Experimental Guidance and Technical Tips

• The immunogen lies within residues 50–140 of human HOXB2, encompassing the conserved homeobox domain. This region’s similarity in mouse and rat underlies the reported cross-reactivity; however, validation in the intended species is advised.
• For western blotting (WB), anticipate a band near 38 kDa corresponding to full-length HOXB2. Use appropriate loading controls and confirm specificity in your sample matrix.
• In ELISA assays, the recombinant protein immunogen can be employed as a positive control or coating antigen. Optimize conditions (e.g., coating buffer, blocking agent) for best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic and research communities by offering a comprehensive portfolio of IVD raw materials. From validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents, we support developmental biology projects centered on HOX gene targets. With one-stop access to rare-target sourcing and technical consultation, CamelBio accelerates your journey from concept to clinic.