Anti-HOXB1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P14653

Core Product Specifications and Parameters

Biological Background: HOXB1 Function and Localization

• HOXB1 (Homeobox protein Hox-B1, also known as Hox-2I) is a homeobox-containing transcription factor encoded by the HOXB1 gene, and alternative splicing may produce multiple isoforms.
• It functions as a sequence-specific transcription factor that provides cells with specific positional identities along the anterior-posterior axis during embryonic development, acting primarily on anterior body structures.
• As a member of the HOX gene family, HOXB1 plays a critical role in body plan patterning and morphogenesis.
• The protein localizes to the nucleus, consistent with its function as a DNA-binding transcriptional regulator.
• HOXB1 contains a homeobox DNA-binding domain, enabling its sequence-specific interaction with target genes.
• Genetic variants in HOXB1 are associated with developmental disorders (UniProt keyword: Disease variant), underscoring its importance in normal embryogenesis.

Experimental Guidance and Technical Tips

• The immunogen (residues 35-180) encompasses a region that may include DNA-binding interfaces; consider verifying reactivity with endogenous full-length HOXB1 in your sample system.
• For Western blot (WB), a band around 32 kDa is expected; validate the antibody in relevant tissue or cell lysates, such as embryonic samples or cultured cells expressing HOXB1.
• In immunofluorescence/immunocytochemistry (IF/ICC), a nuclear staining pattern is anticipated; optimization of fixation and permeabilization conditions may be necessary for your specific samples.
• ELISA can be employed for detection of recombinant or purified HOXB1 protein; ensure proper coating, blocking, and antibody titration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing experience for IVD raw materials, including this anti-HOXB1 polyclonal antibody for developmental biology and transcription research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, supporting your assay development from concept to clinic.